NMR Services for Radio-labeled/Non-labeled/Microgram Samples

In support of our metabolism and Certificate of Analysis programs, XBL provides in-house NMR (Nuclear Magnetic Resonance) services with the addition of a Bruker Avance 500 MHz spectrometer. Traditionally, milligram levels of samples are required to obtain routine NMR spectral data. XBL's new service offering provides detailed NMR analysis from sub milligram samples of non-labeled, or radio-labeled (¹⁴C, ³H) samples, including limited quantities of <10 microgram. In addition, XBL has extensive experience in isolation, purification and identification of metabolites, impurities, and degradation products suitable for NMR and MS analysis.

XBL's comprehensive NMR services include:

1-D and 2-D Analyses and Spectral Data Interpretation

• ¹H, ¹³C, ¹⁹F, ¹⁵N probes
• COSY, NOESY, HMQC, HMBC; other specialized studies
• Sub mg sample analysis, including levels <10µg
• Capillary NMR analysis available for very mass-limited samples

Metabolite Structure Elucidation/Confirmation

• Non-labeled or ¹⁴C and ³H radio-labeled samples
• Bulk metabolite isolation and purification from in-vitro or in-vivo matrices

Impurities/Degradation Products

• HPLC/UV & LC/MS/MS evaluation of impurity or degradation products
• Confirmation of impurity structure by NMR

Certificate of Analyses

• GLP, non-GLP NMR data packages
• Complete C of A services for test articles and reference standards